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Nano LC System

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产品名称: Nano LC System
产品型号: MieuxTM
产品展商: YAMATO
产品文档: 无相关文档
简单介绍
Nano LC System MieuxTM 在产品内容中有关于Nano LC System的详细介绍。


Nano LC System的详细介绍

 

Nano LC System
MieuxTM

Introducing the NewMieux!

Cutting edge pumping technology for outstanding stability andreproducibility

The Mieux System is an innovative system developed for nano-LCfor MS. This system is best suited for proteome, metabolome, anddrug metabolism analyses, as well as for high-separation andhigh-sensitivity analysis.

Gradient pumping exceeding50 nL/min.
Our unique pumping system can achieve a gradient of 50 nL/min.Direct pumping without using the splitter ensures less change inthe flow rate under conditions which produce pressure fluctuations(associated with column exchange), offering stable reproducibilityand makes it unnecessary to adjust the split ratio.

* Features
  • Unique pumping system, enabling a gradient of 50 nL/min withoutusing the splitter
  • Pressure monitor enables full-time monitoring of pumping state
  • Various programs (auto flash, auto refill, auto drain) enablethe System’s high-speed equilibrium and full-automatic pumping
  • Proprietary software enables full-automatic measurement for 1Dto 2D systems
  • Combining this system with the Spotter Mieux SP (OFF Line/LCMS)for MALDI-TOF MS enables spotting to a target plate andmicro-fractionation
  • Connectable to other types of MS
  • Unique nano-spray stage and nano-spray column holder available(optional)
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* Connectable to other mass spectrometers
Applied Biosystems / MDS CIEX · Micromass· BRUKER DALTONICS · Thermo Fisher, and others
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* Analysis data sample (Mieux+Q-Star Elite, t-BSA 50fmol Coverage 70%)
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* Nano-spray stages and nano-spray holders for various MS systems
Nano Spray Stage for LCQ Nano Spray Stage for LTQ Nano Spray Stage Holder for LTQ, Esquire, Accu TOF
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Mieux System
    Configuration
Mieux-I Manual injection system. This system enablesdesalting condensation manually using the trap column. Gradient pump, nano-injection valve withactuator, control software, PC, analytical column, trap column
Mieux-II Auto injection system, which enables gradientanalysis after automatic sample injection and desalting. Gradient pump, single pump, auto sampler,trap column valve, control software, PC, analytical column, trapcolumn (*)
Mieux-III Direct injection system, which has beendeveloped for high-sensitivity detection of phosphoric peptides. Gradient pump, single pump, auto sampler(micro specifications), trap column valve, control software, PC,phosphoric peptide analytical column, trap column (*)
Mieux-IV Two-dimensional auto injection system. Thison-line two-dimensional system performs the single dimension ionexchange in a stepwise manner and two-dimensional reverse-phaseseparation and elution based on the gradient set-up. Gradient pump, single pump, auto sampler,trap column valve, ion exchange column valve, control software, PC,ion exchange column, analytical column, and trap column (*)
Mieux-V Two-dimensional auto injection system. Thison-line two-dimensional system performs analysis based on thegradient set-up in one and two dimensions. Gradient pumps ×2, single pumps ×2, autosampler, trap column valve, ion exchange column valve ×2, controlsoftware, PC, ion exchange column, analytical column, trap column(*)
* Single pump is installed in "Mieux"
• Optional functions available for Phosphoric Peptide Analysisin each systems of Mieux-I, II, IV, and V.
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* User-friendly dedicated software
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* Pumping flow schematic
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* Various Columns
Various column packing, Nano-LC Trap column,Nano-LC capillary column, Micro, semi-micro, conventional,preperative isolation column
Nano-LC capillary column
Size number Column size
2-* 0.2mm I.D × *mmL
1.5-* 0.15mm I.D × *mmL
1-* 0.1mm I.D × *mmL
1-* 0.75-* 0.075mm I.D × *mmL

Nano-LC trap column
Size number Column size
0.5-1 0.5mm I.D × 1mmL
0.8-3 0.8mm I.D × 3mmL
* Same price regardless of size Please ask the typeof column packing and size which you require.
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Mieux Series
Mieux
Gradient Nano Pump
Remarks
Auto injection system : single pump included
Manual injection system : single pump not included (gradient pumponly)
Mieux AS
Auto Sampler
Mieux AP
Single Nano Pump
Mieux SP
MALDI Spotter
Nano Micro-Fraction Collector
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Mieux Spotter System    For MALDI-TOFMS

Example:
1504 spottings on target plate of Applied Bio 4800
By connecting with Mieux,this system enables stable spotting at nL level.

Continuous dispensing to multiple plates
  • Mixed operation of various mfr’s MALDI plates and Microplatesis possible
Simultaneous automatic addition of matrix solution
  • Sample eluate and matrix added simultaneously from the needletip by using the dedicated nano pump (Mieux AP)
  • Plate is not damaged by spotting because a non-contact needleis used
  • Spotting of sample solution is possible at 100 nL/min by usinga dedicated needle
Operation control by dedicated software
  • Integrated control of the SP system together with the MieuxNano LC system
  • Dispensing time can be set freely, starting from 5 seconds
  • Dispensing is possible using an external signal from thedetector
  • Automatic dispensing time calculation function based on theentered analytical time and number of dispensing steps
Configuration Mieux SP-I Mieux-I system, Mieux SP, Mieux AP
Mieux SP-II Mieux-II system, Mieux SP, Mieux AP
Mieux SP-III Mieux-II system, Mieux SP, Mieux AP Mieux-IIIsystem, Mieux SP, Mieux AP
Mieux SP-IV Mieux-IV system, Mieux SP, Mieux AP
Mieux SP-V Mieux-V system, Mieux SP, MieuxAP
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* Specifications
Nano gradient pump(Mieux)
Pumping type One high-pressure double syringe pumpingsystem installed
One high-pressure single syringe pumping system installed
Syringe volume 220uL
Operation control with PC operating dedicated software
Communication RS-232C
Flow rate range High-pressure double syringe pumping type
1 nL/min to 200,000 nL/min in 1 nL step (Gradient 50 nL/min to 1000nL/min)
High-pressure single syringe pumping type
1 nL/min to 200,000 nL/min in 1 nL step
Working pressure 0.0 to 20.0MPa
Pressure indication 0.0 to 50.0MPa in the 0.1 MPa step
Functions Auto flush, auto refill, auto drain
Backside terminal RS-232C, external program start terminal,pressure output (2-ch)
Power supply AC100V to 240V
Size W300 × H210 × D500mm
Power consumption Max 200 VA
Nano pump (MieuxAP)
Pumping type High-pressure syringe pumping system
Syringe volume 220uL
Operation control with PC operating dedicated software
Communication RS-232C
Flow range 1 nL/min to 100,000 nL/min in 1 nL step
Working pressure 0.0 to 15.0Mpa
Pressure indication 0.0 to 50.0MPa in 0.1 MPa step
Functions Auto flush, auto refill, auto drain
Backside terminal RS-232C, external program start terminal,pressure output
Power supply AC100V to 240V
Size W140 × H160 × D320mm
Power consumption Max 150 VA
Auto injector (Mieux AS) (standardspecifications)
Sample injection method Full loop, partial loop, micro pickup
Sample injection rate 0.1 to 100uL
Sample rack 48 vial containers, 96 plates (Low,High), 384(Low)
Injection accuracy Full loop RSD≦0.3
Partial loop RSD≦0.5%(>5μL)
Micro pickup RSD≦1.0%(>5μL)
(*For the micro specifications, contact us separately. )
Operation control With PC operating dedicated software or fromthe keypad on the main body
Communication RS-232C
Cooling function Min 4 deg.C (room temperature -16 deg. C)
Power supply AC115V(50/60Hz), AC230V(50/60Hz)
Size W280 × H400 × D440mm
Power consumption Max. 250VA
*Descriptions in the catalog are subject tochange without notice.
Nano HPLC fraction collector (MieuxSP)
Applicable plate MALDI Plate Applied Biosystems/MDS SCIEX Max 8 plates
Bruker Daltonics Max 4 plates
Waters/Micromass Max 4 plates
Microplate 96 well plate(Low, High), 384 well plate(Low)
Spotting method Contact or non-contact spotting,
Matrix mixed spotting without needle contact
(Note: Matrix fluid feed pump is optional.)
Probe Two-layer (PTFE and SUS) structure
(Sample is center and the matrix solution eluting from the outside)
Allowable dispensing flow range 100nL/min. ~
Matrix flow rate range 100nL/min. ~
Spotting mode Continuous mode (continuous spotting every fixed giveninterval)
Continuous spotting by external pulse signal
Needle Cleaning Automatic needle cleaning by washingsolvent
Waste solution port provided
Spotting direction Straight, zigzagging
Operation control PC operating dedicated software
Communication RS-232C
Power supply AC100 to AC240
Size W500 × H330 × D490mm
Weight Approx. 39 kg
(Note: Matrix fluid feedpump is optional.)

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